Why acetonitrile and methanol are most commonly used solvent for reverse phase HPLC?

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Shaikh Subahan
24-May-2017

Chromatography HPLC Science

Question added by Ankita Gambhir , Lab Specialist , Scientific Diamond Company
Date Posted: 2017/02/26

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1 Answers

by Mohammed Ajmal , Research student , IIRBS

Reverse phase HPLC uses non-polar stationary phase and a polar solvent. Hence it commonly employs more polar methanol and acetonitrile as solvents

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